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Addgene

CMVTO-RBDK65E-nTEVp
(Plasmid #179666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMVTO-FlpIn
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRaf RBD protein
  • Species
    H. sapiens (human)
  • Mutation
    K65E
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Tag / Fusion Protein
    • nTEVP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMVTO-RBDK65E-nTEVp was a gift from Xiaojing Gao (Addgene plasmid # 179666 ; http://n2t.net/addgene:179666 ; RRID:Addgene_179666)

  • For your References section:

    Protease-controlled secretion and display of intercellular signals. Vlahos AE, Kang J, Aldrete CA, Zhu R, Chong LS, Elowitz MB, Gao XJ. Nat Commun. 2022 Feb 17;13(1):912. doi: 10.1038/s41467-022-28623-y. 10.1038/s41467-022-28623-y PubMed 35177637
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