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Addgene

HULAa50H9
(Plasmid #179628)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179628 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HULAa50H9
  • Species
    Actinobacteria
  • Promoter araBAD
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site PmeI (MssI) (not destroyed)
  • 5′ sequencing primer pBAD-Fw 5-ATGCCATAGCATTTTTATCCA-3
  • 3′ sequencing primer pBAD-Rv 5-GATTTAATCTGTATCAGG-3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.02.16.431466 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HULAa50H9 was a gift from Oded Beja (Addgene plasmid # 179628 ; http://n2t.net/addgene:179628 ; RRID:Addgene_179628)

  • For your References section:

    Diverse heliorhodopsins detected via functional metagenomics in freshwater Actinobacteria, Chloroflexi and Archaea. Chazan A, Rozenberg A, Mannen K, Nagata T, Tahan R, Yaish S, Larom S, Inoue K, Beja O, Pushkarev A. Environ Microbiol. 2022 Jan;24(1):110-121. doi: 10.1111/1462-2920.15890. Epub 2022 Jan 4. 10.1111/1462-2920.15890 PubMed 34984789
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