pGEX-DAXX-D/E-His
(Plasmid #179619)

• Purpose: Express DAXX D/E region in bacteria
• Depositing Lab: Xiaolu Yang
• Publication: Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586-021-03824-5. doi: 10.1038/s41586-021-03824-5.

Backbone

• Vector backbone: pGEX-1ZT
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: use BL21(DE3) for protein expression
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: death domain associated protein
• Species: H. sapiens (human)
• Mutation: amino acids 414-505 of DAXX
• Entrez Gene: DAXX (a.k.a. BING2, DAP6, EAP1, SMIM40)
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • TEV (N terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry