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Addgene

pFREE2-SpCas9-sgRNA
(Plasmid #179582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFREE
  • Total vector size (bp) 7490
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFREE2-SpCas9-sgRNA was a gift from Kevin Solomon (Addgene plasmid # 179582 ; http://n2t.net/addgene:179582 ; RRID:Addgene_179582)

  • For your References section:

    Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies. Lee KZ, Mechikoff MA, Parasa MK, Rankin TJ, Pandolfi P, Fitzgerald KS, Hillman ET, Solomon KV. ACS Synth Biol. 2022 Jan 21;11(1):53-60. doi: 10.1021/acssynbio.1c00340. Epub 2022 Jan 10. 10.1021/acssynbio.1c00340 PubMed 35007422
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