pJH2827
(Plasmid #179542)

• Purpose: Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP
• Depositing Lab: Mei Zhen
• Publication: Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.

Backbone

• Vector backbone: pBSK
• Backbone manufacturer: stratagene
• Backbone size w/o insert (bp): 8030
• Total vector size (bp): 8582
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tomm20::miniSOG
• Species: C. elegans (nematode)
• Insert Size (bp): 552
• GenBank ID:
• Entrez Gene: tomm-20 (a.k.a. CELE_F23H12.2)
• Promoter: Pnmr-1
• Tag / Fusion Protein:
  • wCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: atgtcggacacaattc
• 3′ sequencing primer: TCCAGTATTTCATC

Resource Information

• Supplemental Documents:
  • pJH2827.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry