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pJCC_163 SpCas9 K233A/K234A/K253A/K263A
(Plasmid #179527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2CT
  • Backbone manufacturer
    UC Berkeley MacroLab
  • Backbone size w/o insert (bp) 5969
  • Total vector size (bp) 10073
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 K233A/K234A/K253A/K263A
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4104
  • Mutation
    changed lysines 233, 234, 253, and 263 to alanine
  • Tags / Fusion Proteins
    • 10X His (N terminal on backbone)
    • maltose-binding protein (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid 101199 (pSHS207 - Bacterial expression plasmid for WT SpCas9)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJCC_163 SpCas9 K233A/K234A/K253A/K263A was a gift from Jennifer Doudna (Addgene plasmid # 179527 ; http://n2t.net/addgene:179527 ; RRID:Addgene_179527)

  • For your References section:

    CRISPR-Cas9 bends and twists DNA to read its sequence. Cofsky JC, Soczek KM, Knott GJ, Nogales E, Doudna JA. Nat Struct Mol Biol. 2022 Apr;29(4):395-402. doi: 10.1038/s41594-022-00756-0. Epub 2022 Apr 14. 10.1038/s41594-022-00756-0 PubMed 35422516
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