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pET21a Sa-iPGM-bio
(Plasmid #179523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 7059
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. aureus iPGM bio-6His
  • Alt name
    Sa iPGM bio
  • Alt name
    2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
    1616
  • GenBank ID
    WP_020977084.1 AGU54589.1
  • Promoter T7
  • Tags / Fusion Proteins
    • biotinylation sequence (C terminal on insert)
    • His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a Sa-iPGM-bio was a gift from James Inglese (Addgene plasmid # 179523 ; http://n2t.net/addgene:179523 ; RRID:Addgene_179523)

  • For your References section:

    Serum-Stable and Selective Backbone-N-Methylated Cyclic Peptides That Inhibit Prokaryotic Glycolytic Mutases. van Neer RHP, Dranchak PK, Liu L, Aitha M, Queme B, Kimura H, Katoh T, Battaile KP, Lovell S, Inglese J, Suga H. ACS Chem Biol. 2022 Aug 19;17(8):2284-2295. doi: 10.1021/acschembio.2c00403. Epub 2022 Jul 29. 10.1021/acschembio.2c00403 PubMed 35904259
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