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Addgene

MIGR1-Cdc42-2
(Plasmid #179518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIGR1
  • Backbone manufacturer
    Addgene plasmid #27490
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 6800
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cdc42 splice variant 2 cDNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • GenBank ID
    NP_426359
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Promoter LTR
  • Tag / Fusion Protein
    • IRES-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pBabe-5
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid#20141

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIGR1-Cdc42-2 was a gift from Bo Porse (Addgene plasmid # 179518 ; http://n2t.net/addgene:179518 ; RRID:Addgene_179518)

  • For your References section:

    PTBP1 promotes hematopoietic stem cell maintenance and red blood cell development by ensuring sufficient availability of ribosomal constituents. Rehn M, Wenzel A, Frank AK, Schuster MB, Pundhir S, Jorgensen N, Vitting-Seerup K, Ge Y, Jendholm J, Michaut M, Schoof EM, Jensen TL, Rapin N, Sapio RT, Andersen KL, Lund AH, Solimena M, Holzenberger M, Pestov DG, Porse BT. Cell Rep. 2022 May 10;39(6):110793. doi: 10.1016/j.celrep.2022.110793. 10.1016/j.celrep.2022.110793 PubMed 35545054
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