MIGR1-Cdc42-1
(Plasmid
#179517)
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PurposeRetroviral expression of Cdc42 splice variant 1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMIGR1
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Backbone manufacturerAddgene plasmid #27490
- Backbone size w/o insert (bp) 6200
- Total vector size (bp) 6800
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc42 splice variant 1 cDNA
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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GenBank IDNP_001034891
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Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
- Promoter LTR
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Tag
/ Fusion Protein
- IRES-EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBabe-5 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid#20142
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MIGR1-Cdc42-1 was a gift from Bo Porse (Addgene plasmid # 179517 ; http://n2t.net/addgene:179517 ; RRID:Addgene_179517) -
For your References section:
PTBP1 promotes hematopoietic stem cell maintenance and red blood cell development by ensuring sufficient availability of ribosomal constituents. Rehn M, Wenzel A, Frank AK, Schuster MB, Pundhir S, Jorgensen N, Vitting-Seerup K, Ge Y, Jendholm J, Michaut M, Schoof EM, Jensen TL, Rapin N, Sapio RT, Andersen KL, Lund AH, Solimena M, Holzenberger M, Pestov DG, Porse BT. Cell Rep. 2022 May 10;39(6):110793. doi: 10.1016/j.celrep.2022.110793. 10.1016/j.celrep.2022.110793 PubMed 35545054