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Addgene

XLone-BSD NFIL3
(Plasmid #179512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene # 96930
  • Backbone size w/o insert (bp) 5549
  • Total vector size (bp) 6995
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFIL3
  • Alt name
    E4BP4
  • Alt name
    IL3BP1
  • Alt name
    NF-IL3AA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1389
  • Entrez Gene
    NFIL3 (a.k.a. E4BP4, IL3BP1, NF-IL3A, NFIL3A)
  • Promoter TRE3GS Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer gcgcctataaaagagtgctga
  • 3′ sequencing primer cgcctgtcttaggttggagt
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human NFIL3 was cloned from Addgene plasmid #82985, a gift from Jesse Boehm Lab.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-BSD NFIL3 was a gift from Xiaoping Bao (Addgene plasmid # 179512 ; http://n2t.net/addgene:179512 ; RRID:Addgene_179512)

  • For your References section:

    Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells. Jung J, Chang Y, Jin G, Lian X, Bao X. ACS Synth Biol. 2022 May 24. doi: 10.1021/acssynbio.2c00017. 10.1021/acssynbio.2c00017 PubMed 35608547
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