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Addgene

hsLEMD2-pX330
(Plasmid #179511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Backbone size w/o insert (bp) 8484
  • Total vector size (bp) 8518
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA for LEMD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pX330 plasmid was obtained from Addgene (#42230).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hsLEMD2-pX330 was a gift from Yasushi Hiraoka (Addgene plasmid # 179511 ; http://n2t.net/addgene:179511 ; RRID:Addgene_179511)

  • For your References section:

    Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase. Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y. Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8. 10.1038/s42003-022-03021-8 PubMed 35058555
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