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pGFP-LacI
(Plasmid #179510)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-EF1α-MCS-IRES-Neo
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 6892
  • Total vector size (bp) 8700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LacI
  • Species
    E. coli
  • Insert Size (bp)
    1050
  • GenBank ID
    GHK10426.1
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP-LacI was a gift from Yasushi Hiraoka (Addgene plasmid # 179510 ; http://n2t.net/addgene:179510 ; RRID:Addgene_179510)

  • For your References section:

    Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase. Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y. Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8. 10.1038/s42003-022-03021-8 PubMed 35058555
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