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Addgene

EC.RNe.TYB1.a1-395.wt
(Plasmid #17950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB1
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 7280
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Escherichia coli RNase E residues 1-395
  • Species
    E. coli
  • Insert Size (bp)
    1185
  • Mutation
    residues 1-395
  • Tag / Fusion Protein
    • intein-chitin binding domain (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Stan Cohen, Stanford

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC.RNe.TYB1.a1-395.wt was a gift from David McKay (Addgene plasmid # 17950 ; http://n2t.net/addgene:17950 ; RRID:Addgene_17950)

  • For your References section:

    Retention of core catalytic functions by a conserved minimal ribonuclease E peptide that lacks the domain required for tetramer formation. Caruthers JM, Feng Y, McKay DB, Cohen SN. J Biol Chem. 2006 Sep 15. 281(37):27046-51. 10.1074/jbc.M602467200 PubMed 16854990
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