Skip to main content
Addgene

HA-FOXO3a TM delta DB
(Plasmid #1795)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1795 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pECE
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXO3a
  • Alt name
    FKHRL1
  • Alt name
    forkhead family
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    T32A, S253A, S315A, delta DB (deletion of nucleotides 446 to 689)
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Tag / Fusion Protein
    • HA (YDVPDYASL) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations in phosphorylation sites and deletion of DNA binding domain. See HA-FOXO3a WT delta DB for wild-type map and sequence.
Can be used instead of 1793: HA-FOXO3a TM DBM (H212R); has the same biological function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-FOXO3a TM delta DB was a gift from Michael Greenberg (Addgene plasmid # 1795)

  • For your References section:

    DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS, Chiang LW, Greenberg ME. Science 2002 Apr 19;296(5567):530-4. 10.1126/science.1068712 PubMed 11964479
Related items: