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pHybrid-Electra2
(Plasmid #179475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHybrid
  • Backbone size w/o insert (bp) 4895
  • Total vector size (bp) 5609
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Electra2
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    714
  • GenBank ID
    OL631607

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGAGAATTCACGCGTGGTACCAGCCACCATGGTGTCTAAGGGCGAAGAGC
  • 3′ sequencing primer GATGCCTGGGTCGACTCTAGATTACTTGAACAGCTCGTCCATGCCAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mScarlet-I
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    696

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHybrid-Electra2 was a gift from Kiryl Piatkevich (Addgene plasmid # 179475 ; http://n2t.net/addgene:179475 ; RRID:Addgene_179475)

  • For your References section:

    Dual-expression system for blue fluorescent protein optimization. Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD. Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. 10.1038/s41598-022-13214-0 PubMed 35715437
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