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pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo
(Plasmid #179439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB (PiggyBac)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H2B-mIFP-T2A-rTetR-KRAB
  • Alt name
    H2B-mIFP, T2A cleavage site, rTetR-KRAB(human)
  • Species
    H. sapiens (human), Synthetic
  • Promoter pGK

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo was a gift from Lacra Bintu (Addgene plasmid # 179439 ; http://n2t.net/addgene:179439 ; RRID:Addgene_179439)

  • For your References section:

    Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, Fujimori T, Bintu L. Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115. 10.7554/eLife.75115 PubMed 35678392
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