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pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
(Plasmid #179436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAVS1 donor
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HS4-TRE-cit-HS4-mch (DH)
  • Alt name
    TRE, H2B-mCitrine, chicken HS4 insulators, H2B-mCherry
  • Species
    H. sapiens (human), G. gallus (chicken), Synthetic
  • Promoter pEF alpha, pRSV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry was a gift from Lacra Bintu (Addgene plasmid # 179436 ; http://n2t.net/addgene:179436 ; RRID:Addgene_179436)

  • For your References section:

    Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, Fujimori T, Bintu L. Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115. 10.7554/eLife.75115 PubMed 35678392
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