pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
(Plasmid #179431)

• Purpose: Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
• Depositing Lab: Lacra Bintu
• Publication: Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.

Backbone

• Vector backbone: pSLR-test
• Backbone manufacturer: Yamaguchi et al. 2011 PLoS One, doi: 10.1371/journal.pone.0017267
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRE-cit-coreHS4-mch (SC)
• Alt name: TRE, H2B-mCitrine, core chicken HS4 insulator, H2B-mCherry
• Species: H. sapiens (human), G. gallus (chicken), Synthetic
• Promoter: pEF alpha, pRSV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.