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pv6_MECP2_MBD-T158M
(Plasmid #179410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    parBit_v6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MECP2_MBD(T158M)
  • Alt name
    MBD domain of MECP2
  • Species
    M. musculus (mouse)
  • Mutation
    MBD T158M
  • Entrez Gene
    Mecp2 (a.k.a. 1500041B07Rik, D630021H01Rik, Mbd5, WBP10)
  • Promoter CAGGS
  • Tags / Fusion Proteins
    • AviTag (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pv6_MECP2_MBD-T158M was a gift from Tuncay Baubec (Addgene plasmid # 179410 ; http://n2t.net/addgene:179410 ; RRID:Addgene_179410)

  • For your References section:

    ChromID identifies the protein interactome at chromatin marks. Villasenor R, Pfaendler R, Ambrosi C, Butz S, Giuliani S, Bryan E, Sheahan TW, Gable AL, Schmolka N, Manzo M, Wirz J, Feller C, von Mering C, Aebersold R, Voigt P, Baubec T. Nat Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 10.1038/s41587-020-0434-2. 10.1038/s41587-020-0434-2 PubMed 32123383
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