pv7_MBD1_MBD_noEGFP
(Plasmid
#179406)
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PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179406 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneparBit_v7
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMBD1_MBD
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Alt nameMBD domain of MBD1, 2A cleavage peptide-EGFP
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SpeciesM. musculus (mouse)
- Promoter CAGGS
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Tag
/ Fusion Protein
- AviTag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CTGGTTGTTGTGCTGTCTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pv7_MBD1_MBD_noEGFP was a gift from Tuncay Baubec (Addgene plasmid # 179406 ; http://n2t.net/addgene:179406 ; RRID:Addgene_179406) -
For your References section:
ChromID identifies the protein interactome at chromatin marks. Villasenor R, Pfaendler R, Ambrosi C, Butz S, Giuliani S, Bryan E, Sheahan TW, Gable AL, Schmolka N, Manzo M, Wirz J, Feller C, von Mering C, Aebersold R, Voigt P, Baubec T. Nat Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 10.1038/s41587-020-0434-2. 10.1038/s41587-020-0434-2 PubMed 32123383