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Addgene

pET-epELM-5
(Plasmid #179391)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28C (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5367
  • Total vector size (bp) 5504
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB C3013
  • Growth instructions
    Depositor recommends NEB C3013 for downstream applications.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELMSAN1
  • Alt name
    MIDEAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    153
  • GenBank ID
    NM_194278.3
  • Entrez Gene
    MIDEAS (a.k.a. C14orf117, C14orf43, ELMSAN1, LSR68, c14_5541)
  • Promoter T7 lac
  • Tag / Fusion Protein
    • 6x His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-epELM-5 was a gift from Shiro Iuchi (Addgene plasmid # 179391 ; http://n2t.net/addgene:179391 ; RRID:Addgene_179391)

  • For your References section:

    RNAmetasome network for macromolecule biogenesis in human cells. Iuchi S, Paulo JA. Commun Biol. 2021 Dec 15;4(1):1399. doi: 10.1038/s42003-021-02928-y. 10.1038/s42003-021-02928-y PubMed 34912035
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