pLenti-mCherry-Cre-blast
(Plasmid #179390)

• Purpose: Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
• Depositing Lab: Moshe Szyf
• Publication: Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi: 10.1038/s41467-021-25991-9.

Backbone

• Vector backbone: pLenti6/V5
• Backbone size w/o insert (bp): 6488
• Total vector size (bp): 9175
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry (P2A) Cre recombinase
• Species: H. sapiens (human), M. musculus (mouse), R. norvegicus (rat)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pLM-CMV-R-Cre (Addgene Plasmid #27546)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The plasmid contains an IS1 element insertion within a region of the plasmid near the origin of replication and outside of the region that will be packaged as part of the lentivirus particles to be produced from this plasmid. This insertion is not known to affect plasmid function.