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Addgene

pGEX6P1-hPREP
(Plasmid #179387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P-1
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human prolyl oligopeptidase
  • Alt name
    POP
  • Alt name
    PREP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2133
  • GenBank ID
    NM_002726.4
  • Entrez Gene
    PREP (a.k.a. PE, PEP)
  • Promoter TAC
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGGCTGGCAAGCCACGTTTGGTG
  • 3′ sequencing primer GGAGCTGCATGTGTCAGAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1-hPREP was a gift from Christian Gruber (Addgene plasmid # 179387 ; http://n2t.net/addgene:179387 ; RRID:Addgene_179387)

  • For your References section:

    Discovery of a Beetroot Protease Inhibitor to Identify and Classify Plant-Derived Cystine Knot Peptides. Retzl B, Hellinger R, Muratspahic E, Pinto MEF, Bolzani VS, Gruber CW. J Nat Prod. 2020 Nov 25;83(11):3305-3314. doi: 10.1021/acs.jnatprod.0c00648. Epub 2020 Oct 29. 10.1021/acs.jnatprod.0c00648 PubMed 33118348
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