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Addgene

pCDH-TfR-mApple
(Plasmid #179384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7644
  • Total vector size (bp) 10695
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transferrin receptor 1 - mApple
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3039
  • GenBank ID
    7037
  • Entrez Gene
    TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
  • Promoter EF1
  • Tag / Fusion Protein
    • mApple (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer caagctgtgaccggcg
  • 3′ sequencing primer gcttcggccagtaacgtta
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mApple and TfR DNA were obtained from mApple-Lysosomes-20 (RRID:Addgene_54921) and mCherry-TFR-20 (RRID:Addgene_55144) respectively, which were a gift from Michael Davidson.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.04.13.488146 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-TfR-mApple was a gift from Angus Johnston (Addgene plasmid # 179384 ; http://n2t.net/addgene:179384 ; RRID:Addgene_179384)

  • For your References section:

    Resolving subcellular pH with a quantitative fluorescent lifetime biosensor. Rennick JJ, Nowell CJ, Pouton CW, Johnston APR. Nat Commun. 2022 Oct 12;13(1):6023. doi: 10.1038/s41467-022-33348-z. 10.1038/s41467-022-33348-z PubMed 36224168
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