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pTSB-FICAT_Payload-Esp3I
(Plasmid #179380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB512
  • Backbone size (bp) 4972
  • Modifications to backbone
    Removed mcs, EF1a RFP to include Esp3I restriction sites for cloning of any desired gene for targeted delivery
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golden gate piggyback transposon backbone to clone transgene for targeted transgene insertion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTSB-FICAT_Payload-Esp3I was a gift from Marc Güell (Addgene plasmid # 179380 ; http://n2t.net/addgene:179380 ; RRID:Addgene_179380)

  • For your References section:

    Find and cut-and-transfer (FiCAT) mammalian genome engineering. Pallares-Masmitja M, Ivancic D, Mir-Pedrol J, Jaraba-Wallace J, Tagliani T, Oliva B, Rahmeh A, Sanchez-Mejias A, Guell M. Nat Commun. 2021 Dec 3;12(1):7071. doi: 10.1038/s41467-021-27183-x. 10.1038/s41467-021-27183-x PubMed 34862378
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