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pET28a(+)- PC-MjMAT
(Plasmid #179372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PC-MjMAT
  • Alt name
    MjMAT (L147A, I351A)
  • Species
    Methanocaldococcus jannaschii
  • Mutation
    L147A, I351A
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+)- PC-MjMAT was a gift from Andrea Rentmeister (Addgene plasmid # 179372 ; http://n2t.net/addgene:179372 ; RRID:Addgene_179372)

  • For your References section:

    Visible-Light Removable Photocaging Groups Accepted by MjMAT Variant: Structural Basis and Compatibility with DNA and RNA Methyltransferases. Peters A, Herrmann E, Cornelissen NV, Klocker N, Kummel D, Rentmeister A. Chembiochem. 2021 Oct 4. doi: 10.1002/cbic.202100437. 10.1002/cbic.202100437 PubMed 34606675
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