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Addgene

pLKO.1-PPP2R3B-1
(Plasmid #179360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PR48
  • gRNA/shRNA sequence
    CGAGCATTTCTACGTCATCTA
  • Species
    H. sapiens (human)
  • Promoter Age1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer LKO.1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-PPP2R3B-1 was a gift from William Hahn (Addgene plasmid # 179360 ; http://n2t.net/addgene:179360 ; RRID:Addgene_179360)

  • For your References section:

    Identification of PP2A complexes and pathways involved in cell transformation. Sablina AA, Hector M, Colpaert N, Hahn WC. Cancer Res. 2010 Dec 15;70(24):10474-84. doi: 10.1158/0008-5472.CAN-10-2855. 10.1158/0008-5472.CAN-10-2855 PubMed 21159657
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