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Addgene

pGEX4T1-WWP1F792D
(Plasmid #179328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T-1
  • Backbone manufacturer
    GEHealthcare
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WWP1 F792D
  • Alt name
    AIP5
  • Alt name
    Tiul1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2783
  • Mutation
    F792D mutation; Codon Optimized for expression in E. coli
  • Entrez Gene
    WWP1 (a.k.a. AIP5, Tiul1, hSDRP1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1-WWP1F792D was a gift from Arthur Haas (Addgene plasmid # 179328 ; http://n2t.net/addgene:179328 ; RRID:Addgene_179328)
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