Skip to main content
Addgene

pGEX4T1-Opt WWP1
(Plasmid #179327)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX4T-1
  • Backbone manufacturer
    GEHealthcare
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WWP1
  • Alt name
    AIP5
  • Alt name
    Tiul1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2783
  • Mutation
    Codon optimized for expression in E. coli
  • Entrez Gene
    WWP1 (a.k.a. AIP5, Tiul1, hSDRP1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1-Opt WWP1 was a gift from Arthur Haas (Addgene plasmid # 179327 ; http://n2t.net/addgene:179327 ; RRID:Addgene_179327)
Related items:
Commonly requested with: