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Addgene

pEGFP-mCCK2R-stop
(Plasmid #179321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Total vector size (bp) 6076
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse cholecystokinin2 receptor
  • Alt name
    CCK2R
  • Alt name
    CCKBR
  • Species
    M. musculus (mouse)
  • Mutation
    stop codon after ORF
  • GenBank ID
    NM_007627
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GAGGTCTATATAAGCAGAGC
  • 3′ sequencing primer ACTTGTGGCCGTTTACGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-mCCK2R-stop was a gift from Christian Gruber (Addgene plasmid # 179321 ; http://n2t.net/addgene:179321 ; RRID:Addgene_179321)

  • For your References section:

    Discovery of the cyclotide caripe 11 as a ligand of the cholecystokinin-2 receptor. Taghizadeh MS, Retzl B, Muratspahic E, Trenk C, Casanova E, Moghadam A, Afsharifar A, Niazi A, Gruber CW. Sci Rep. 2022 Jun 2;12(1):9215. doi: 10.1038/s41598-022-13142-z. 10.1038/s41598-022-13142-z PubMed 35654807
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