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Addgene

pEYFP-NR1a
(Plasmid #17928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    N-methyl-D-aspartate receptor NMDAR1
  • Alt name
    NMDAR1-1a
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4914
  • GenBank ID
    U08261
  • Entrez Gene
    Grin1 (a.k.a. GluN1, NMDAR1, NR1)
  • Tag / Fusion Protein
    • EYFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-NR1a was a gift from Stefano Vicini (Addgene plasmid # 17928 ; http://n2t.net/addgene:17928 ; RRID:Addgene_17928)

  • For your References section:

    Functional expression of distinct NMDA channel subunits tagged with green fluorescent protein in hippocampal neurons in culture. Luo JH, Fu ZY, Losi G, Kim BG, Prybylowski K, Vissel B, Vicini S. Neuropharmacology. 2002 Mar . 42(3):306-18. 10.1016/S0028-3908(01)00188-5 PubMed 11897109

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