pEGFP-NR1a
(Plasmid #17926)

• Depositing Lab: Stefano Vicini
• Publication: Luo et al Neuropharmacology. 2002 Mar . 42(3):306-18.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A
• Alt name: NR1a
• Species: R. norvegicus (rat)
• Insert Size (bp): 4914
• GenBank ID: U08261
• Entrez Gene: Grinl1a (a.k.a. Glurr)
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original NR1a was a generous gift of Dr. Jim Boulter (University of California, Los Angeles) and it was subcloned by Dennis R Grayson now at Univ of Illinois.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry