pEGFP-NR2B
(Plasmid #17925)

• Depositing Lab: Stefano Vicini
• Publication: Luo et al Neuropharmacology. 2002 Mar . 42(3):306-18.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4716
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: N-methyl D-aspartate receptor 2B
• Alt name: NR2B
• Species: R. norvegicus (rat)
• Insert Size (bp): 5291
• GenBank ID:
• Entrez Gene: Grin2b (a.k.a. GluN2B)
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS identified a few sequence discrepancies relative to Genbank ID: M91562, but the plasmid is expected to function as described in the associated publication.