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Addgene

pJH2140
(Plasmid #179249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSK
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 9228
  • Total vector size (bp) 10389
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UNC-9
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1161
  • GenBank ID
  • Entrez Gene
    unc-9 (a.k.a. CELE_R12H7.1)
  • Promoter Pacr-5
  • Tag / Fusion Protein
    • wCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CGgatccggATGAGTATGCTATT
  • 3′ sequencing primer CGGGgatccTCACACGTCGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH2140 was a gift from Mei Zhen (Addgene plasmid # 179249 ; http://n2t.net/addgene:179249 ; RRID:Addgene_179249)

  • For your References section:

    An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion. Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M. Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005. 10.1016/j.neuron.2011.09.005 PubMed 22099460
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