PGEX-DDX21-D2+C
(Plasmid
#179243)
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Purposeprotein purification in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179243 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDDX21-D2+C
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SpeciesH. sapiens (human)
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Mutationaa396-782
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Entrez GeneDDX21 (a.k.a. GUA, GURDB, II/Gu, RH, RH II/Gu, RH-II/GU, RH-II/GuA, gu-alpha)
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Tag
/ Fusion Protein
- GST-flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGEX-DDX21-D2+C was a gift from Ling-Ling Chen (Addgene plasmid # 179243 ; http://n2t.net/addgene:179243 ; RRID:Addgene_179243) -
For your References section:
lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription. Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang ZH, Shan L, Yang L, Liu J, Chen LL. Science. 2021 Jul 30;373(6554):547-555. doi: 10.1126/science.abf6582. 10.1126/science.abf6582 PubMed 34326237