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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA1.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNMDAR2A
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Alt nameNR2A
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SpeciesR. norvegicus (rat)
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Insert Size (bp)5150
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GenBank IDD13211
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Entrez GeneGrin2a (a.k.a. GluN2A, NMDAR2A, NR2A)
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer sequence (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-NR2A was a gift from Stefano Vicini (Addgene plasmid # 17924 ; http://n2t.net/addgene:17924 ; RRID:Addgene_17924) -
For your References section:
Functional expression of distinct NMDA channel subunits tagged with green fluorescent protein in hippocampal neurons in culture. Luo JH, Fu ZY, Losi G, Kim BG, Prybylowski K, Vissel B, Vicini S. Neuropharmacology. 2002 Mar . 42(3):306-18. 10.1016/S0028-3908(01)00188-5 PubMed 11897109
Map uploaded by the depositor.
