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p23-mNeongreen-DDX21 WT-mRuby3
(Plasmid #179225)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179225 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-EF1a-IRES-ZsGreen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX21
  • Species
    H. sapiens (human)
  • Entrez Gene
    DDX21 (a.k.a. GUA, GURDB, II/Gu, RH, RH II/Gu, RH-II/GU, RH-II/GuA, gu-alpha)
  • Tags / Fusion Proteins
    • mNeongreen (N terminal on insert)
    • mRuby3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p23-mNeongreen-DDX21 WT-mRuby3 was a gift from Ling-Ling Chen (Addgene plasmid # 179225 ; http://n2t.net/addgene:179225 ; RRID:Addgene_179225)

  • For your References section:

    lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription. Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang ZH, Shan L, Yang L, Liu J, Chen LL. Science. 2021 Jul 30;373(6554):547-555. doi: 10.1126/science.abf6582. 10.1126/science.abf6582 PubMed 34326237
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