pJH2069
(Plasmid #179224)

• Purpose: Pnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry
• Depositing Lab: Mei Zhen
• Publication: Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.

Backbone

• Vector backbone: pBSK
• Backbone manufacturer: stratagene
• Backbone size w/o insert (bp): 8030
• Total vector size (bp): 9191
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UNC-9
• Species: C. elegans (nematode)
• Insert Size (bp): 1161
• GenBank ID:
• Entrez Gene: unc-9 (a.k.a. CELE_R12H7.1)
• Promoter: Pnmr-1
• Tag / Fusion Protein:
  • wCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CGACGgatccggATGAGTATGCTATTG
• 3′ sequencing primer: ATCTCGGGgatccTCACACGTCGT

Resource Information

• Supplemental Documents:
  • pJH2069.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry