pYPQ134-tRNA
(Plasmid
#179213)
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Purpose(Empty Backbone) Golden Gate entry vector; 4th gRNA with tRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179213 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHD1
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Backbone manufacturerCermak et al. 2011
- Backbone size (bp) 3600
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Vector typeCRISPR
- Promoter no promoter
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ134-tRNA was a gift from Yiping Qi (Addgene plasmid # 179213 ; http://n2t.net/addgene:179213 ; RRID:Addgene_179213) -
For your References section:
Boosting plant genome editing with a versatile CRISPR-Combo system. Pan C, Li G, Malzahn AA, Cheng Y, Leyson B, Sretenovic S, Gurel F, Coleman GD, Qi Y. Nat Plants. 2022 May;8(5):513-525. doi: 10.1038/s41477-022-01151-9. Epub 2022 May 20. 10.1038/s41477-022-01151-9 PubMed 35596077