pJH2157
(Plasmid
#179204)
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PurposePacr-2::Ca -insensitive D3cpv unc-54 3' UTR C.elegans neural expression of Pacr-2 calcium D3cpv
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBSK
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Backbone manufacturerstratagene
- Backbone size w/o insert (bp) 6992
- Total vector size (bp) 8951
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameD3cpv
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Insert Size (bp)1959
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GenBank ID
- Promoter Pacr-2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CGgatcccatggtgagcaaggg
- 3′ sequencing primer GGAATTcttactcgatgttgt (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH2157 was a gift from Mei Zhen (Addgene plasmid # 179204 ; http://n2t.net/addgene:179204 ; RRID:Addgene_179204) -
For your References section:
An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion. Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M. Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005. 10.1016/j.neuron.2011.09.005 PubMed 22099460