Skip to main content
Addgene

pJH1863
(Plasmid #179200)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 179200 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pBSK
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 6992
  • Total vector size (bp) 8951
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D3cpv
  • Insert Size (bp)
    1959
  • GenBank ID
  • Promoter Pacr-2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CGgatcccatggtgagcaagggcg
  • 3′ sequencing primer TTGGAATTcttactcgatgttgtgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH1863 was a gift from Mei Zhen (Addgene plasmid # 179200 ; http://n2t.net/addgene:179200 ; RRID:Addgene_179200)

  • For your References section:

    An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion. Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M. Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005. 10.1016/j.neuron.2011.09.005 PubMed 22099460
Related items: