Skip to main content
Addgene

pCAG-mPpp3cc-FLAG-6XHIS
(Plasmid #179160)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCAG1.1
  • Backbone size w/o insert (bp) 5160
  • Total vector size (bp) 6770
  • Modifications to backbone
    pCAGGS was used as an original vector.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    protein phosphatase 3, catalytic subunit, gamma isoform
  • Alt name
    Ppp3cc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1610
  • Entrez Gene
    Ppp3cc (a.k.a. Calnc, PP2BA gamma)
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • 6XHIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GCCTTCTTCTTTTTCCTACAGC
  • 3′ sequencing primer GCCACACCAGCCACCACCTTCTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mPpp3cc-FLAG-6XHIS was a gift from Masahito Ikawa (Addgene plasmid # 179160 ; http://n2t.net/addgene:179160 ; RRID:Addgene_179160)

  • For your References section:

    Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Miyata H, Satouh Y, Mashiko D, Muto M, Nozawa K, Shiba K, Fujihara Y, Isotani A, Inaba K, Ikawa M. Science. 2015 Oct 23;350(6259):442-5. doi: 10.1126/science.aad0836. Epub 2015 Oct 1. 10.1126/science.aad0836 PubMed 26429887
Related items: