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Addgene

PB-3-FT-ENGRAM
(Plasmid #179158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBac-Puro
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Csy4
  • Species
    Pseudomonas aeruginosa
  • Promoter minP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGGGATACGGGGAAAAGGCCTCCACGGCCA
  • 3′ sequencing primer AACTAAACTTGTTTATTGCAGCTTATAATGGTTACAAATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-3-FT-ENGRAM was a gift from Jay Shendure (Addgene plasmid # 179158 ; http://n2t.net/addgene:179158 ; RRID:Addgene_179158)

  • For your References section:

    Symbolic recording of signalling and cis-regulatory element activity to DNA. Chen W, Choi J, Li X, Nathans JF, Martin B, Yang W, Hamazaki N, Qiu C, Lalanne JB, Regalado S, Kim H, Agarwal V, Nichols E, Leith A, Lee C, Shendure J. Nature. 2024 Aug;632(8027):1073-1081. doi: 10.1038/s41586-024-07706-4. Epub 2024 Jul 17. 10.1038/s41586-024-07706-4 PubMed 39020177
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