pADV-Sec61b-TurboID
(Plasmid #179143)

• Purpose: Recombinant adenoviral plasmid for Sec61b-TurboID expression
• Depositing Lab: Jae Myoung Suh
• Publication: Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi: 10.1038/s41467-021-25546-y.

Backbone

• Vector backbone: pAdTrack-CMV, pAdEasy-1
• Backbone manufacturer: Tong-Chuan He laboratory
• Backbone size w/o insert (bp): 35725
• Total vector size (bp): 37036
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sec61b-V5-TurboID
• Species: Synthetic
• Insert Size (bp): 1311
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • pADV-Sec61b-TurboID.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sec61b-V5-TurboID_pcDNA5 is Addgene plasmid # 166971.

To generate adenovirus, digest with PacI and perform transfection to HEK293 cells according to Luo et al (PMID:17546019) procedure #14.

Please note: The plasmid contains a 1650bp insertion just downstream of the Left Arm compared to the depositor's provided sequence. This difference is not known to affect plasmid function.