SnapTag-Binder
(Plasmid
#179142)
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PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4868
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSspB
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Alt nameBinder
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SpeciesHaemophilus influenzae
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Insert Size (bp)912
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MutationY11K/A15E, residue numbering from pdb: 1ou9
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Tag
/ Fusion Protein
- SNAP-tag (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SnapTag-Binder was a gift from Klaus Hahn (Addgene plasmid # 179142 ; http://n2t.net/addgene:179142 ; RRID:Addgene_179142) -
For your References section:
Biosensors based on peptide exposure show single molecule conformations in live cells. Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm JB, Lavis LD, Elston TC, Hahn KM. Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 10.1016/j.cell.2021.09.026. Epub 2021 Oct 11. 10.1016/j.cell.2021.09.026 PubMed 34637702
