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Addgene

Flag-mCherry-Binder
(Plasmid #179139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6105
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SspB
  • Alt name
    Binder
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    1092
  • Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • FLAG (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-mCherry-Binder was a gift from Klaus Hahn (Addgene plasmid # 179139 ; http://n2t.net/addgene:179139 ; RRID:Addgene_179139)

  • For your References section:

    Biosensors based on peptide exposure show single molecule conformations in live cells. Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm JB, Lavis LD, Elston TC, Hahn KM. Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 10.1016/j.cell.2021.09.026. Epub 2021 Oct 11. 10.1016/j.cell.2021.09.026 PubMed 34637702
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