pHAGE N' EGFP NAP1 FL IRES Blast
(Plasmid
#179100)
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PurposeExpression of full length NAP1 cDNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
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Backbone manufacturerJ. Wade Harper's Laboratory
- Backbone size w/o insert (bp) 8340
- Total vector size (bp) 9519
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNak associated protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1179
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Entrez GeneAZI2 (a.k.a. AZ2, NAP1, TILP)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMFf
- 3′ sequencing primer MSCVr (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE N' EGFP NAP1 FL IRES Blast was a gift from Alicia Pickrell (Addgene plasmid # 179100 ; http://n2t.net/addgene:179100 ; RRID:Addgene_179100) -
For your References section:
NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. Paul S, Sarraf SA, Nam KH, Zavar L, DeFoor N, Biswas SR, Fritsch LE, Yaron TM, Johnson JL, Huntsman EM, Cantley LC, Ordureau A, Pickrell AM. J Cell Biol. 2024 Feb 5;223(2):e202303082. doi: 10.1083/jcb.202303082. Epub 2023 Dec 7. 10.1083/jcb.202303082 PubMed 38059900