pRDA_479
(Plasmid #179099)

• Purpose: BE Cas expression
• Depositing Labs: John Doench, David Root
• Publication: Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi: 10.1038/s41467-022-28884-7.

Backbone

• Vector backbone: pRDA_426
• Vector type: Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABE8e (SpG)
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pRDA_479.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry