pTU422
(Plasmid #178982)

• Purpose: 13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)
• Depositing Lab: Stan Brouns
• Publication: van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353. doi: 10.1126/science.abk2718. Epub 2021 Aug 26.

Backbone

• Vector backbone: p13S-S
• Backbone size w/o insert (bp): 4021
• Total vector size (bp): 9977
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: gRAMP
• Species: Candidatus Scalindua brodae
• Insert Size (bp): 5169
• Promoter: T7
• Tag / Fusion Protein:
  • Strep-Tag II SUMO (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGGG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Gene/Insert 2

• Gene/Insert name: CRISPR array
• Species: Candidatus Scalindua brodae
• Insert Size (bp): 787
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAACGTGATGGTAAAATGG
• 3′ sequencing primer: ACAATTCGTTCAAGCCGAGGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry