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Addgene

pHN922
(Plasmid #178975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Cytiva
  • Total vector size (bp) 5320
  • Vector type
    Bacterial Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GST-His8-GFP-nanobody
  • Species
    Synthetic
  • Insert Size (bp)
    355
  • Promoter tac
  • Tag / Fusion Protein
    • GST, His8 (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTTTATACATGGACCCAATGTGC
  • 3′ sequencing primer ATCCGCTTACAGACAAGCTGTGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHN922 was a gift from Kazuhiro Abe (Addgene plasmid # 178975 ; http://n2t.net/addgene:178975 ; RRID:Addgene_178975)

Ready-to-use antibodies

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